| Solution Information | help | |
| Enzyme: | Transitional endoplasmic reticulum ATPase | |
| inhibitor: | BDBM47020 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this assay is to determine dose response curves for 16 compounds synthesized to explore structure activity relationship of SID 14723044, which was identified as active in a previous set of experiments entitled, "Primary biochemical high-throughput screening assay to measure p97 ATPase inhibition" (PubChem AID 1481), and confirmed activity in a subsequent set of experiments entitled, "Confirmation biochemical high-throughput screening assay for inhibitors of the p97 ATPase" (PubChem AID 1517). The 16 synthesized compounds were tested in a previous assay entitled, "Luminescence dose response biochemical high throughput screening assay for inhibitors of the p97 ATPase: synthesized compounds," (PubChem AID 1551). The target of the present assay is mutant Cys522Ala p97 protein and thus serves as a counterscreen for AID 1551. This biochemical assay employs the Kinase-Glo reagent, which contains a luciferase that emits luminescence in direct proportion to ATP | |
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